Prrsv Antibody Elisa Test Kit - China

Porcine Reproductive and Respiratory Syndrome Virus ( PRRSV)

Antibody ELISA kit

Catalog No.

LSY- 30010

1 - Brief

The GreenSpring® Porcine reprodutive and respiratory syndrome virus antibody test kit ( PRRSV Ab) is used for the detection of PRRSV antibodies in swine serum; assessment of immunity conditions against porcine reprodutive and respiratory syndrome virus in the pig farms; and investigation epidemiology of the PRRSV.

2 - Principle

The GreenSpring® PRRSV Ab ELISA test kit is made from the antigen coated microtiter plate, goat- anti- pig IgG- HRP and other reagents.

It applies the indirect ELISA principle to test the antibodies against PRRSV in porcine serum.

In the test, the coated antigen combine with PRRSV- IgG in serum, then add IgG- HRP to specifically bind with complex of antibody- antigens on the microplate.

With the TMB substrate, it will generate an amount of color.

The depth of color is relative with the content of the PRRSV- IgG.

3 - Components

1 PRRSV Antigen Coated Microplate 2 plates ( 96 wells)

2 PRRSV- IgG Negative control ( green lid) 1 tube ( 1.5 mL)

3 PRRSV- IgG Positive control ( red lid) 1 tube ( 1.5 mL)

4 PRRSV Enzyme Conjugate ( yellow lid) 1 bottle ( 22 mL)

5 Wash Concentrate ( 20×) ( white lid) 1 bottle ( 50 mL)

6 Substrate A ( purple lid) / B ( orange lid) 1 bottle each ( 12 mL/ bottle)

7 Stop solution ( blue lid) 1 bottle ( 12 mL)

8 Sample diluent solution ( transparent lid) 1 bottle ( 50 mL)

9 Adhesive Foil 6 pieces

10 Instruction 1 piece

( Note: Never mix up reagents from test kits in different batch number)

4 - Material Required not Provided

1 Microplate Reader ( single- wave length: 450nm or double- wave length: 450/ 630 nm) .

2 Microplate Washer.

3 Micropipettes, adjustable.

4 Constant temperature box or water bath box.

5 Oscillator.

5 - Sample requirement

1 The samples are porcine serum, which should be collected with no bacteria.

The storage time should be less than 1 week at 2- 8 ℃ , if for long term, it should be kept at - 20℃ .

2 Avoid to use the samples with severe hemolysis, precipitate, contaminated by bacteria or protein suspension.

3 The EDTA, heparin sodiun and other anticoagulants will not affect the results.

Company Contact:

• Posted By: Shenzhen Lvshiyuan Biotechnology Co.,Ltd

• Phone: 8675528938075

• Address: D building, National Biological Industrial Park of, Shenzhen , China

• Email:

• Website: https://www.lsybt.com

• Business Description: involved in international trade import export of goods, products, and services