Porcine Encephalitis Virus Elisa Test Kit - China

Porcine Japanese B Encephalitis Virus Ab ELISA Kit

Catalog No.

LSY-30008

1 - Usage

The porcine encephalitis virus ELISA test kit is used for the detection of the porcine encephalitis virus antibodies in swine serum; assessment the immunity conditions against porcine encephalitis virus in the pig farms and investigation of the epidemiology of the porcine encephalitis virus.

2 - Principle

The kit is based on an indirect enzymatic immunoassay(Indirect ELISA).

The antigen is coated on plates.

When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates.

Wash the unbound antibodies and other components.

Then add a specific peroxidase conjugate(IgG-HRP).

After incubation and washing, add the TMB substrate.

A colorimetric reaction will appear, measured by a spectrophotometer(630 nm).

3 - The kit components

1 Antigen coated microtiter plate 2 pieces (96 wells)

2 Negative (green lid)/positive (red lid)serum 1 tube each (1.5 mL/tube)

3 Enzyme conjugate (yellow lid) 1 bottle (25 mL/bottle)

4 20×concentrated washing buffer (white lid) 1 bottle (50 mL/bottle)

5 Substrate A (purple lid)/B (yellow lid) 1 bottle each (12 mL/bottle)

6 Stop solution(blue lid) 1 bottle (12 mL/bottle)

7 Sample diluent solution(transparent lid) 1 bottle (50 mL/bottle)

8 Adhesive Foil 6 pieces

9 Instruction 1 piece

4 - Materials Required But Not Provided

1 Microplate Reader (double-wave length: 450/630 nm).

2 Precise micropipette (single-channel 1-100ul, 0.5-10ul, multi-channel 30-300ul)

3 Constant temperature box or water bath box.

4 Oscillator.

5 Microplate Washer.

6 Disposable tips (10ul, 200ul)

7 Deionized water

Company Contact:

• Posted By: Shenzhen Lvshiyuan Biotechnology Co.,Ltd

• Phone: 8675528938075

• Address: D building, National Biological Industrial Park of, Shenzhen , China

• Email:

• Website: https://www.lsybt.com

• Business Description: involved in international trade import export of goods, products, and services