Aflatoxins B1 Elisa Test Kit - China

Aflatoxins B1 (AFB1) ELISA Test Kit

Catalog No.

LSY-10028

1 - Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Aflatoxins B1 - The coupling antigen is pre-coated on the micro-well stripes.

The Aflatoxins B1 in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Aflatoxins B1 antibodies.

After the addition of the enzyme conjugate, the TMB substrate is added for coloration.

The optical density (OD) value of the sample has a negative correlation with the Aflatoxins B1 in the sample.

This value is compared to the standard curve and the Aflatoxins B1 residues is subsequently obtained.

2 - Technical specifications

Sensitivity: 0.03 ppb

Incubator temperature: 25℃

Incubator time: 30min～15min

Detection limit:

Milk (Method 1).

…….

0.15ppb

Milk (Method 2)…………………†¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦ ….

………….

0 .

3ppb

Tissue.

0.72ppb

Feed .

…0.9ppb

Urine.

….

….

0.06ppb

Cross-reaction rate:

Aflatoxins B1… …….

100%

Recovery rate:

Milk….

… 90±23%

Tissue….

….

85±18%

Feed, urine………………†¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦ ……….

…………… ….

85±21%

3 - Components

1) Micro-well strips: 12 strips with 8 removable wells each

2) 6× standard solution (1 mL each): 0 ppb, 0.03 ppb, 0.06 ppb, 0.12 ppb, 0.24 ppb , 0.48 ppb

3) Enzyme conjugate (7 mL) red cap

4) Antibody working solution (7 mL) blue cap

5) Substrate A solution (7 mL) white cap

6) Substrate B solution (7 mL) black cap

7) Stop solution (7 mL) yellow cap

8) 20× concentrated washing buffer (40 mL) white cap

9) 2× concentrated redissolving solution (50 mL) transparent cap

Company Contact:

• Posted By: Shenzhen Lvshiyuan Biotechnology Co.,Ltd

• Phone: 8675528938075

• Address: D building, National Biological Industrial Park of, Shenzhen , China

• Email:

• Website: https://www.lsybt.com

• Business Description: involved in international trade import export of goods, products, and services