Human Ube2a Ubiquitin Conjugating Enzyme E2a Elisa Kit - China

Product name

Human UBE2A ( Ubiquitin Conjugating Enzyme E2A) ELISA Kit

Catalog No.



E- EL- H2381

Package

96T/ kit

Detection Range

0.313- 20ng/ mL

Sensitivity

0.188 ng/ m

L

Specificity

This kit recognizes natural and recombinant Human UBE2A.

No significant cross- reactivity or interference between Human UBE2A and analogues was observed.

Note： Limited by existing techniques, cross- reaction may still exist, as it is impossible for us to complete the cross- reactivity detection between Human UBE2A and all the analogues.

Repeatability

Coefficient of variation were<10% .

Test principle

This ELISA kit uses Sandwich- ELISA as the method.

The micro ELISA plate provided in this kit has been pre- coated with an antibody specific to Human UBE2A.

Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody.

Then a biotinylated detection antibody specific for Human UBE2A and Avidin- Horseradish Peroxidase ( HRP) conjugate is added to each micro plate well successively and incubated.

Free components are washed away.

The substrate solution is added to each well.

Only those wells that contain Human UBE2A, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color.

The enzyme- substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow.

The optical density ( OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.

The OD value is proportional to the concentration of Human UBE2A.

You can calculate the concentration of Human UBE2A in the samples by comparing the OD of the samples to the standard curve.

Company Contact:

• Posted By: Wuhan Elabscience Biotechnology Co., Ltd

• Phone: 862787385095

• Address: No 1, Shizishan Street, Hongshan District , Wuhan , China

• Email:

• Website: https://www.elabscience.com

• Business Description: Elabscience ELISA Kits supplier offering 20, 000 kind of
  
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