Human Pon3 Paraoxonase 3 Elisa Kit - China

Product name

Human PON3 ( Paraoxonase 3) ELISA Kit

Other name

PON3

Catalog No.



E- EL- H2374

Package

96T/ kit

Detection Range

0.234- 15ng/ mL

Sensitivity

0.141 ng/ mL

Specificity

This kit recognizes natural and recombinant Human PON3 - No significant cross- reactivity or interference between Human PON3 and analogues was observed.

Note： Limited by existing techniques, cross- reaction may still exist, as it is impossible for us to complete the cross- reactivity detection between Human PON3 and all the analogues.

Repeatability

Coefficient of variation were<10% .

Test principle

This ELISA kit uses Sandwich- ELISA as the method.

The micro ELISA plate provided in this kit has been pre- coated with an antibody specific to Human PON3 - Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody.

Then a biotinylated detection antibody specific for Human PON3 and Avidin- Horseradish Peroxidase ( HRP) conjugate is added to each micro plate well successively and incubated.

Free components are washed away.

The substrate solution is added to each well.

Only those wells that contain Human PON3, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color.

The enzyme- substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow.

The optical density ( OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.

The OD value is proportional to the concentration of Human PON3 - You can calculate the concentration of Human PON3 in the samples by comparing the OD of the samples to the standard curve.

Company Contact:

• Posted By: Wuhan Elabscience Biotechnology Co., Ltd

• Phone: 862787385095

• Address: No 1, Shizishan Street, Hongshan District , Wuhan , China

• Email:

• Website: https://www.elabscience.com

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