946 Agonists Elisa Test Kit - China

1 - Principle

The BIOKAU®β - agonists test kit is based on the competitive

enzyme immunoassay for the detection of β - agonists in the

urine, pork and other samples.

The coupling antigen is pre-

coated on the micro- well stripes.

The β - agonists in the

sample and the coupling antigens pre- coated on the micro-

well stripes compete for the anti- β - agonists antibodies.

After the addition of the enzyme conjugate, the TMB

substrate is added for coloration.

The optical density ( OD)

value of the sample has a negative correlation with the β -

agonists in the sample.

This value is compared to the

standard curve and the β - agonists residues is subsequently

obtained.

2 - Technical specifications

Sensitivity: 0.3 ppb

Detection limit:

Tissue

0.3 ppb

Urine,

serum.

Feed

.

30 ppb

Recovery rate:

90±10%

80±10%

80±15%

Cross- reaction rate:

Clenbuterol.

.

100%

Salbutamol

120%

Bromchlorbuterol

.

130%

Brombuterol.

.

90%

Mabuterol.

.

70%

Clenpenterol

65%

Mapenterol

55%

Tebutalin.

42%

Carbuterol

.

35%

Clenproperol

.

15%

Cimaterol.

12%

Dobutamine

<3%

Ritodrine.

<1%

Ractopamine.

.

<0.5%

Precision:

For the intra- assay precision, CV<8%

For the inter- assay precision, CV<10%

3 - Components

1） Micro- well strips: 12 strips with 8 removable wells

each

2） 6× standard solution ( 1 mL each) : 0 ppb, 0.3ppb, 0.9

ppb, 2.7 ppb, 8.1 ppb, 25 ppb

3） Enzyme conjugate ( 7

mL) .

.

.

.

.

.

.

red cap

4） Antibody working solution ( 10

mL) blue cap

5） Substrate A solution ( 7

mL) .

white cap

6） Substrate B solution ( 7

black cap

7） Stop solution ( 7

.

.

.

.

.

.

.

.

.

yellow cap

8） 20× concentrated washing buffer ( 40

mL) .

white cap

9） 2× concentrated redissolving solution ( 50

mL) .

transparent cap

4 - Materials required but not provided

1) Equipments: microplate reader, printer, homogenizer,

nitrogen- drying device, vortex, oscillator, centrifuge,

measuring pipets, balance ( a sensibility reciprocal of 0.01

g)

2) Micropipettors: single- channel 20- 200 µL, 100- 1000

µL, and multi- channel 250 µL;

3) Reagents: Acetonitrile ( CH3CN) , NaOH, ethyl acetate,

HCI ( approx 36.5% ) , N- hexane, isopropanol( C3H8O)

5 - Sample pre- treatment

Solution preparation before sample pre- treatment:

1) 1 M HCI: dissolve 8.6 mL HCI ( approx 36.5% ) in

deionized water to 100 mL.

2) 1 M NaOH: dissolve 4 g NaOH in deionized water to

100 mL.

3) Dillute 2× concentrated redissolving solution with

deionized water at 1: 1( 1× concentrated redissolving

solution, used for sample redissolving) and 1: 3( 0.5×

concentrated redissolving solution, used for extraction of

tissue sample)

Company Contact:

• Posted By: Shenzhen Biokau Sci-tech Co.,ltd

• Phone: 86013724287324

• Address: Rm206, Building A DongJin Block, Zhongshan Road, S, Shenzhen , China

• Email:

• Website: http://www.biokau.com

• Business Description: We are a team of experienced scientists with many years of experience on
  
  antigen, antibody, Gold Colloid and ELISA kit.
  
  We offer you a wide range of research reagents, food safety rapid test kits, animal disease diagnostic kits, antibodies and rapid strips, both unique and routine.
  
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