Recombinant Proteinase K - China

Recombinant Proteinase K

CAS NO: 39450-01-6

EC NO: 3.4.21.14

1 - Product introduction Proteinase K is a non-specific endoproteinase, which is a serine protease that can cleave ester bonds and peptide bonds connected to the carboxyl ends of aliphatic amino acids, hydrophobic amino acids and aromatic amino acids.

The molecular weight is 28.9 kDa (monomer).

It is active at pH 4 to pH 12 - It is also stable in the presence of SDS, urea or EDTA.

It can cleave the carboxy terminal peptide bonds of aliphatic and aromatic amino acids, and is used for the degradation of proteins in biological samples.

This enzyme has been decolorized and purified by chromatography to remove RNA and DNA, and the activity of other miscellaneous enzymes can not be detected.

Because proteinase K is stable in urea and SDS, and has the ability to degrade natural proteins, the smallest polypeptide that is hydrolyzed is a tetrapeptide molecule.

2 - Product advantages High purity: no other miscellaneous enzyme activity: no other side reactions in the digestion reaction, high yield

High specific activity: specific activity not less than 30 u/mg protein Electrophoresis purity: identified by SDS-PAGE electrophoresis, over 99% Stable: freeze-dried powder, easy to store and transport 3 - Product use Mainly used in genetic diagnosis kits, genomic DNA extraction kits, RNA extraction kits to remove nucleases in DNA and RNA preparation, and can also be used to extract non-protein components from tissues to degrade protein-containing impurities, such as DNA vaccines and heparin Preparation, etc., can also prepare chromosomal DNA for pulse electrophoresis and Western blotting.

4 - Product features Source: Tritirachium album limber

Company Contact:

• Posted By: Shanghai Kekai Biotechnology Co., Ltd.

• Phone: 15000107241

• Address: Room 723, Building 3, No. 268, Zhouzhu Road, Shanghai International Medical College, SHANGHAI , SHANGHAI , China

• Email:

• Website: http://www.koka-bio.com

• Business Description: Shanghai Kekai Biotechnology Co., Ltd. is mainly engaged in the R&D and production of hyaluronidase, recombinant trypsin, recombinant trypsin, recombinant carboxypeptidase B, recombinant streptococcal protein A, recombinant enterokinase, recombinant streptococcal protein G, recombinant chymotrypsin, recombinant protease K, Staphylococcus aureus V8 protease, recombinant KEX protease, recombinant lysine endonuclease, penicillin enzyme, etc