Recombinant Enterokinase Escherichia Coli - China
price: 150.00 Dollar US$
CAS: 9014-74-8
Common factors affecting enterokinase activity
At >200mM Imi, or >200mMNaCl, or >5% glycerol, the digestion effect will be affected.
You can refer to the following recommended methods for digestion:
1) In order to obtain an ideal digestion result, please dialyze the sample into 25mM Tris-HCl 8.0 buffer, and then perform digestion.
2) If it is inconvenient for dialysis, you can dilute the sample.
The imi content is below 100mM, the NaCl concentration is below 50mM, and the glycerol concentration is below 5% for digestion, and the ratio of enzyme dosage to protein remains unchanged (ie 1U digestion 50μg protein) .
3) If the sample solution contains one or more of the above components and it is inconvenient to remove, at this time, increase the enzyme amount or extend the digestion time to achieve better digestion results.
Company Contact:
- Posted By: Shanghai Kekai Biotechnology Co., Ltd.
- Phone: 15000107241
- Address: Room 723, Building 3, No. 268, Zhouzhu Road, Shanghai International Medical College, SHANGHAI , SHANGHAI , China
- Email:
- Website: http://www.koka-bio.com
Published date: October 31, 2022
- Business Description: Shanghai Kekai Biotechnology Co., Ltd. is mainly engaged in the R&D and production of hyaluronidase, recombinant trypsin, recombinant trypsin, recombinant carboxypeptidase B, recombinant streptococcal protein A, recombinant enterokinase, recombinant streptococcal protein G, recombinant chymotrypsin, recombinant protease K, Staphylococcus aureus V8 protease, recombinant KEX protease, recombinant lysine endonuclease, penicillin enzyme, etc
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