Human Interleukin 1beta Elisa Kit - China

Method：sandwich

Detection range：20pg/L→6000pg/L

Sensitivity：10.07pg/ L

Assay length：1-5Hours

Detection Wavelengt：450nm

Research Area：Immunology

Price：inquiry

Quality guarantee period：for 12 months

Introduction

Reagents

Quantity

Reagents

Quantity

Coated ELISA plate

12-Well * 8 Tubes

Washing concentrate (30X)

20ml

Standard dilution

3ml

Instruction

1

Chromogen solution A

6ml

Seal plate membrane

2

Chromogen solution B

6ml

Hermetic bag

1

Streptavidin-HRP

6ml

Stop solution

6ml

Standard solution

0.5ml

Antibodies labeled with biotin

1ml

INTENDED USE

The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

STORAGE

For unopened kit: All the reagents should be kept according to the labels on vials, Washing concentrate (30X) and the Stop Solution should be stored at 4℃ upon receipt while the others should be at -20℃

PRECISION

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<8%

Inter-Assay: CV<10%

ASSAY PROCEDURE SUMMARY

1 - Prepare all reagents, samples and standards;

2 - Add prepared samples, standards and ELISA solutions.

Let them react for 60 minutes at 37 ℃.

3 - Wash the plate five times.

Add Chromogen solution A and B.

Incubate for 10 minutes at 37 ℃; for color development.

4 - Add stop solution

5 - Read the OD value within 10minutes.

6 - Calculate

Company Contact:

• Posted By: Shanghai YL Biotech Co. Ltd

• Phone: 8615057279866

• Address: No.5588,Caoan Road,Jiading District,Shanghai,China, Shanghai , China

• Email:

• Website: https://www.ylbiont.com

• Business Description: YL Biont is a collection of research, production, sales as one of the
  
  leading international biological high-tech enterprise.
  
  the company focused on the immune detection reagents of independent research and development, the main products include ELISA kit, CLIA kit, protein, antibody, mark kit and other related reagents, which a total of more than 20000 products! Through the national support and new high-tech industries promoted, we has been the advanced production technology in the field of biological kits.
  
  YL company will continue to research and develop, improve competition of products quality.
  
  At the same time, the products of the research and development face the market at home and abroad.