Chloramphenicol Elisa Test Kit - China

1 - Principle

This test kit is based on the competitive enzyme immunoassay

for the detection of Chloramphenicol in the sample.

The

coupling antigen is pre- coated on the micro- well stripes.

The Chloramphenicol in the sample and the coupling antigen

pre- coated on the micro- well stripes compete for the anti-

Chloramphenicol antibody.

After the addition of the enzyme

conjugate, the TMB substrate is added for coloration.

The

optical density ( OD) value of the sample has a negative

correlation with the Chloramphenicol in it.

This value is

compared to the standard curve and the Chloramphenicol

concentration is subsequently obtained.

2 - Technical specifications

Sensitivity: 0.05 ppb

Detection limit:

Intestine.

0.1 ppb

Honey.

.

0.15 ppb

Milk

0.05 ppb

Urine,

serum.

Chicken/ liver, pork/ liver, fish,

shrimp.

0.05

ppb

Recovery rate

shrimp

80±10%

.

70±10%

85±15%

Cross- reaction rate:

Chloramphenicol.

.

100%

Thiamphenicol.

< 0.1%

Florfeniol

.

< 0.1%

3 - Components

1） Micro- well strips: 12 strips with 8 removable wells

each

2） 6× standard solution ( 1 mL each) : 0 ppb, 0.05 ppb,

0.15 ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb

3） Enzyme conjugate ( 12

mL) .

.

.

.

.

red cap

4） Concentrated antibody working solution ( 1

mL) blue cap

5） Substrate A solution ( 7

.

white cap

6） Substrate B solution ( 7

mL) .

black cap

7） Stop solution ( 7

.

.

.

.

.

.

.

.

.

yellow cap

8） 20× concentrated washing buffer ( 40

mL) .

white cap

9） 2× concentrated redissolving solution ( 50

mL) .

transparent cap

Company Contact:

• Posted By: Shenzhen Biokau Sci-tech Co.,ltd

• Phone: 86013724287324

• Address: Rm206, Building A DongJin Block, Zhongshan Road, S, Shenzhen , China

• Email:

• Website: http://www.biokau.com

• Business Description: We are a team of experienced scientists with many years of experience on
  
  antigen, antibody, Gold Colloid and ELISA kit.
  
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