Porcine Circovirus Pcv2 Antibody Elisa Test Kit - China

1 - Usage

The porcine circovirous ELISA Test kit is used for detection of porcine circovirus antibodies in the serum; assessment of immunity conditions against porcine circovirus in the pig farm and investigation of the epidemiology of the porcine circovirus.

2 - Principle

The PCV2 ELISA kit is based on an indirect enzymatic immunoassay( Indirect ELISA) .

The antigen is coated on plates.

When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates.

Wash the unbound antibodies and other components.

Then add a specific peroxidase conjugate( IgG- HRP) .

After incubation and washing, add the TMB substrate.

A colorimetric reaction will appear, measured by ELISA Reader.

3 - The kit components

1 PCV Antigen coated microtiter plate 2 pieces ( 96 wells)

2 Negative ( green lid) / positive ( red lid) control 1 tube each ( 1.5 mL/ tube)

3 Enzyme conjugate ( yellow lid) 1 bottle ( 22 mL/ bottle)

4 20×concentrated washing buffer ( white lid) 1 bottle ( 50 mL/ bottle)

5 Substrate A ( purple lid) / B ( orange lid) 1 bottle each ( 12 mL/ bottle)

6 Stop solution( blue lid) 1 bottle ( 12 mL/ bottle)

7 Sample diluent solution( transparent lid) 1 bottle ( 50 mL/ bottle)

8 Adhesive Foil 6 pieces

9 Instruction 1 piece

( Note: Never mix up reagents from test kits in different batch number)

4 - Material Required But not Provided

1Microplate Reader( Dual- wave length: 450/ 630 nm) .

2Microplate Washer.

3Micropipettes, adjustable.

4Constant temperature box or water bath box.

5Oscillator.

Company Contact:

• Posted By: Shenzhen Biokau Sci-tech Co.,ltd

• Phone: 86013724287324

• Address: Rm206, Building A DongJin Block, Zhongshan Road, S, Shenzhen , China

• Email:

• Website: http://www.biokau.com

• Business Description: We are a team of experienced scientists with many years of experience on
  
  antigen, antibody, Gold Colloid and ELISA kit.
  
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