Metronidazole Elisa Kit - China

Metronidazole ELISA kit

Catalog No.

LSY- 10048

1 - Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Metronidazole in the sample.

The coupling antigens are pre- coated on the micro- well stripes.

The Metronidazole in the sample and the coupling antigens pre- coated on the micro- well stripes compete for the anti- Metronidazole antibodies.

After the addition of the enzyme conjugate, the TMB substrate is added for coloration.

The optical density ( OD) value of the sample has a negative correlation with the Metronidazole in it.

This value is compared to the standard curve and the Metronidazole concentration is subsequently obtained.

2 - Technical specifications

Sensitivity: 0.1 ppb

Incubation Temperature: 25℃

Incubation Time: 60min～ 20min~15min

Detection limit

Tissue 0.5 ppb

Honey 0.1 ppb

Cross- reaction rate

Metronidazole 100%

Hydroxy- nitroimidazole 42%

Tinidazole 2.6%

Dimetridazole 80%

Hydroxymethyl nitroimidazole 50%

Ornidazole 6.3%

Recovery rate

Tissue 90±20%

Honey 85±10%

3 - Components

1） Micro- well strips: 12 strips with 8 removable wells each

2） 6× standard solution ( 1 mL each) : 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb

3） Enzyme conjugate ( 7 mL) red cap

4） Antibody working solution ( 7 mL) blue cap

5） Substrate A solution ( 7 mL) white cap

6） Substrate B solution ( 7 mL) black cap

7） Stop solution ( 7 mL) yellow cap

8） 20× concentrated washing buffer ( 40 mL) white cap

9） 2× concentrated redissolving solution ( 50 mL) transparent cap

10） 2- Nitrobenzaldehyde ( C7H5NO3) ( 10 mL) white cap

Company Contact:

• Posted By: Shenzhen Lvshiyuan Biotechnology Co.,Ltd

• Phone: 8675528938075

• Address: D building, National Biological Industrial Park of, Shenzhen , China

• Email:

• Website: https://www.lsybt.com

• Business Description: involved in international trade import export of goods, products, and services