Diethylstilbestrol Elisa Test Kit - China

1 - Principle

The BIOKAU® Diethylstilbestrol ELISA Test Kit is based on

the competitive enzyme immunoassay for the detection of

Diethylstilbestrol ( DES) in the feed, urine, liver, meat,

shrimp and fish.

The conjugate antigen is pre- coated on the

micro- well stripes.

The Diethylstilbestrol in the sample

competes with the conjugate antigen pre- coated on the micro-

well stripes, to interact with the antibodies against

Diethylstilbestrol.

After the addition of the enzyme

conjugate, the TMB substrate is added for coloration.

The

optical density ( OD) value of the sample has a negative

correlation with the content of Diethylstilbestrol in the

sample.

This value is compared to the standard curve and the

content of the corresponding Diethylstilbestrol is

subsequently obtained.

2 - Technical specifications

Sensitivity: 0.1 ppb

Detection limit

Shrimp,

fish

0.2 ppb

Pork/ liver,

chicken/ liver.

.

2 ppb

Urine.

0.6 ppb

Feed

20 ppb

Recovery rate

.

70 ±10%

90 ±10%

Tissue

85 ±10%

Cross- reactions rate

DES.

.

100%

Dienestrol

.

38.5%

Hexestrol.

.

8.5%

Ethinylestradiol

< 0.1%

Estriol.

.

< 0.1%

3 - Components

1） Micro- well strips: 12 strips with 8 removable wells

each

2） 6 × standard solution ( 1 mL each) : 0 ppb, 0.1 ppb,

0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb

3） Enzyme conjugate ( 12

mL) .

.

.

.

.

red cap

4） Antibody working solution ( 7

mL) blue

cap

5） Substrate A solution ( 7

.

white cap

6） Substrate B solution ( 7

mL) .

black cap

7） Stop solution ( 7

.

.

.

.

.

.

.

.

.

yellow cap

8） 20× concentrated washing buffer ( 40

mL) .

white cap

9） 5× concentrated redissolving solution ( 50

mL) .

transparent cap

4 - Materials required but not provided

1) Equipments: microplate reader, printer, homogenizer,

nitrogen- drying device, votex, centrifuge, measuring pipets,

balance ( a sensibility reciprocal of 0.01 g)

2) Micropipettors: single- channel 20- 100 µL , 100- 1000

µL, and multi- channel 250 µL;

3) Reagents: NaOH, Acetonitrile ( CH3CN) , Acetone,

deionized water, H3PO4 ( 85% ) , CHCl3

Company Contact:

• Posted By: Shenzhen Biokau Sci-tech Co.,ltd

• Phone: 86013724287324

• Address: Rm206, Building A DongJin Block, Zhongshan Road, S, Shenzhen , China

• Email:

• Website: http://www.biokau.com

• Business Description: We are a team of experienced scientists with many years of experience on
  
  antigen, antibody, Gold Colloid and ELISA kit.
  
  We offer you a wide range of research reagents, food safety rapid test kits, animal disease diagnostic kits, antibodies and rapid strips, both unique and routine.
  
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  We are proud to be recognized as one of Chinese leading suppliers, and equally proud of our reputation for excellent customer service.
  
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